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Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
Atlantic horseshoe crab Limulus polyphemus. Limulus amebocyte lysate (LAL) is an aqueous extract of motile blood cells from the Atlantic horseshoe crab Limulus polyphemus.LAL reacts with bacterial endotoxins such as lipopolysaccharides (LPS), which are components of the bacterial capsule, the outermost membrane of cell envelope of gram-negative bacteria.
Structure of a lipopolysaccharide (LPS) Lipopolysaccharide, now more commonly known as endotoxin, [1] is a collective term for components of the outermost membrane of the cell envelope of gram-negative bacteria, such as E. coli and Salmonella [2] with a common structural architecture.
The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...
hok/sok homologues denoted flmA/B (FlmA is the protein toxin and FlmB RNA the antisense regulator) [14] are carried on the F plasmid which operate in the same way to maintain the stability of the plasmid. [15] The F plasmid contains another homologous toxin-antitoxin system called srnB. [11]
Gel extraction kits are available from several major biotech manufacturers for a final cost of approximately 1–2 US$ per sample. Protocols included in these kits generally call for the dissolution of the gel-slice in 3 volumes of chaotropic agent at 50 °C, followed by application of the solution to a spin-column (the DNA remains in the column), a 70% ethanol wash (the DNA remains in the ...
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