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The TLC plate can either be dipped in or sprayed with a stain and sometimes heated depending on the stain used. Many stains exist for a large range of chemical moieties but some examples include: [7] [16] [17] Potassium permanganate (no heating, for oxidisable groups) Ninhydrin (heating, amines and amino-acids) Acidic vanillin (heating, general ...
It is necessary to use either a chiral stationary phase or; a chiral additive in the mobile phase. The chiral stationary phase can be prepared by mixing chirally pure reagents such as L-amino acid, or brucine, or a chiral ligand exchange reagent with silica gel slurry, [2] [3] or by impregnation of the TLC plate in the solution of a chiral ...
Thin layer chromatography (TLC) is a quick alternative to more complex chromatography methods. TLC can be used to analyze inks and dyes by extracting the individual components. [18] This can be used to investigate notes or fibers left at the scene since each company's product is slightly different and those differences can be seen with TLC.
Le Roux's research team assessed HPTLC for determining salbutamol serum levels in clinical trials and concluded that it is a suitable method for analyzing serum samples. [3] HPTLC has also been used successfully in the separation of various lipid subclasses, with reproducible and promising results obtained for 20 different lipid subclasses.
It is widely used in analytical chemistry; though the high temperatures used in GC make it unsuitable for high molecular weight biopolymers or proteins (heat denatures them), frequently encountered in biochemistry, it is well suited for use in the petrochemical, environmental monitoring and remediation, and industrial chemical fields. It is ...
A list of chemical analysis methods with acronyms. A Atomic absorption spectroscopy (AAS) ... Gas chromatography (GC) Gas chromatography-mass spectrometry (GC-MS)
Thin-layer chromatography, a chromatography technique used in chemistry to separate chemical compounds Total lung capacity , refers to the volume of air associated with different phases of the respiratory cycle
Paper chromatography is an analytical method used to separate colored chemicals or substances. It can also be used for colorless chemicals that can be located by a stain or other visualisation method after separation. [ 1 ]