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HCV genome. Nonstructural protein 5B (NS5B) is a viral protein found in the hepatitis C virus (HCV). [1] It is an RNA-dependent RNA polymerase, having the key function of replicating HCV's viral RNA by using the viral positive RNA strand as a template to catalyze the polymerization of ribonucleoside triphosphates (rNTP) during RNA replication.
The virus is internalized and the nucleocapsid is released into the cytoplasm of the hepatocyte after binding with the receptor. The NS5B protein, which is an RNA-dependent RNA polymerase, catalyzes hepatitis C virus replication. [10] The hepatitis C virus can cause acute infection but most patients are asymptomatic upon exposure.
It is also possible that NS5A is a critical component during HCV replication and subcellular localization, which may shed light on the poorly understood HCV life cycle. [ 1 ] [ 4 ] Additionally, NS5A has been shown to modulate the polymerase activity of NS5B , an RNA-dependent RNA polymerase (RdRp) . [ 3 ]
The first protease inhibitor approved by the U.S. Food and Drug Administration (FDA). Ritonavir: Norvir: AbbVie: U.S. patent 5,541,206: March 1, 1996: AbbVie was part of Abbott Laboratories when patent was granted. As well as being a protease inhibitor in its own right, ritonavir inhibits the breakdown of other protease inhibitors.
Current research is focused on small-molecule inhibitors of the viral protease, RNA polymerase and other nonstructural genes. Two agents— boceprevir by Merck [ 66 ] and telaprevir by Vertex Pharmaceuticals —both inhibitors of NS3 protease were approved for use on May 13, 2011, and May 23, 2011, respectively.
Daclatasvir, sold under the brand name Daklinza, is an antiviral medication used in combination with other medications to treat hepatitis C (HCV). [5] The other medications used in combination include sofosbuvir, ribavirin, and interferon, vary depending on the virus type and whether the person has cirrhosis. [3] It is taken by mouth. [5]
It acts as an RNA-dependent RNA polymerase inhibitor. It is a prodrug which is converted inside the body to the active form, 2'-C-methylcytidine triphosphate. [1] [2] ...
PCR inhibitors are any factor which prevent the amplification of nucleic acids through the polymerase chain reaction (PCR). [1] PCR inhibition is the most common cause of amplification failure when sufficient copies of DNA are present. [2] PCR inhibitors usually affect PCR through interaction with DNA or interference with the DNA polymerase.