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  2. Western blot - Wikipedia

    en.wikipedia.org/wiki/Western_blot

    Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]

  3. Western blot normalization - Wikipedia

    en.wikipedia.org/wiki/Western_blot_normalization

    Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.

  4. Molecular-weight size marker - Wikipedia

    en.wikipedia.org/wiki/Molecular-weight_size_marker

    Upon completion, a detection mechanism such as western blotting can be used, which will reveal the presence of bands. Each band represents a specific protein. The distance of travel is solely based on molecular weight; therefore, the molecular weight of each protein can be determined by comparing the distance of an unknown protein to the ...

  5. Quantitative proteomics - Wikipedia

    en.wikipedia.org/wiki/Quantitative_proteomics

    It is generally accepted, that while label-free quantification is the least accurate of the quantification paradigms, it is also inexpensive and reliable when put under heavy statistical validation. There are two different methods of quantification in label-free quantitative proteomics: AUC (area under the curve) and spectral counting.

  6. Lyme disease - Wikipedia

    en.wikipedia.org/wiki/Lyme_disease

    Tests for antibodies in the blood by ELISA and Western blot is the most widely used method for Lyme diagnosis. A two-tiered protocol is recommended by the Centers for Disease Control and Prevention (CDC): the sensitive ELISA test is performed first, and if it is positive or equivocal, then the more specific Western blot is run. [116]

  7. Image Studio Lite - Wikipedia

    en.wikipedia.org/wiki/Image_Studio_Lite

    Image Studio™ Lite is free image processing software used for quantitation of Western blot images and images from related experiments. As of 2021, Image Studio Lite has been discontinued and is no longer available for download. In its place, LICOR has released Empiria Studio, a commercial product.

  8. Polyacrylamide gel electrophoresis - Wikipedia

    en.wikipedia.org/wiki/Polyacrylamide_gel...

    Following electrophoresis, the gel may be stained (for proteins, most commonly with Coomassie brilliant blue R-250 or autoradiography; for nucleic acids, ethidium bromide; or for either, silver stain), allowing visualization of the separated proteins, or processed further (e.g. Western blot). After staining, different species biomolecules ...

  9. Bradford protein assay - Wikipedia

    en.wikipedia.org/wiki/Bradford_protein_assay

    The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution.