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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium. [10] The technique of plant tissue culture, i.e., culturing plant cells or tissues in artificial medium supplemented with required nutrients, has many applications in efficient clonal propagation (true to the ...
In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and/or increase the number of cells or microorganisms in the culture. [1]
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
And after the plantlet develops in vitro, then the ex-planting process can often be very stressful and result in plant death, which isn't a problem for pre-acclimated plantlets grown via photoautotrophic tissue culture (In this context, pre-acclimated refers to plantlets that have developed a cuticle and have been cultivated in an open air ...