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CAMP test is shown at bottom left. The CAMP test (Christie–Atkins–Munch-Petersen) is a test to identify group B β-hemolytic streptococci (Streptococcus agalactiae) [1] [2] based on their formation of a substance, CAMP factor, [3] that enlarges the area of hemolysis formed by the β-hemolysin elaborated from Staphylococcus aureus.
The reverse CAMP test utilizes the synergetic hemolytic abilities of the CAMP factor produced by Streptococcus agalactiae with the α-toxin produced by Clostridium perfringens. Streaking these two organisms perpendicular to each other on a blood agar plate will yield a “bow-tie” clearing of the blood agar by the hemolytic capabilities of ...
Streptococcus agalactiae (also known as group B streptococcus or GBS) is a gram-positive coccus (round bacterium) with a tendency to form chains (as reflected by the genus name Streptococcus). It is a beta- hemolytic , catalase -negative, and facultative anaerobe .
Considering that a reliable screening test is more important than a rapid and less accurate result. ASM also states that it is acceptable to use NAAT-based identification of GBS from an enrichment broth (after 18-24-hour incubation) with high sensitivity, and that FDA-cleared commercial assays are available to perform the test. [8]
This phenomenon is the mechanism behind the CAMP test, [2] a test that was historically used for the identification of Streptococcus agalactiae and Listeria monocytogenes. [3] A modified version of this test called the reverse CAMP test, utilizing S. agalactiae instead of S. aureus, can also be used to identify Clostridium perfringens.
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Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England setting. Main Streptococcus groups are included as "Strep." at bottom left. Diagnosis is by a swab of the affected area for laboratory testing.
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