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  2. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    HPLC has many applications in both laboratory and clinical science. It is a common technique used in pharmaceutical development, as it is a dependable way to obtain and ensure product purity. [59] While HPLC can produce extremely high quality (pure) products, it is not always the primary method used in the production of bulk drug materials. [60]

  3. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Preparative HPLC apparatus. Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography.

  4. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    Silica gel particles are commonly used as a stationary phase in high-performance liquid chromatography (HPLC) for several reasons, [13] [14] including: High surface area: Silica gel particles have a high surface area, allowing direct interactions with solutes or after bonding of variety of ligands for versatile interactions with the sample molecules, leading to better separations.

  5. File:Preparative HPLC.svg - Wikipedia

    en.wikipedia.org/wiki/File:Preparative_HPLC.svg

    English: Preparative HPLC system. Based on Agilent 1200 apparatus. Date: 24 July 2010: Source: Own work: Author: G YassineMrabet Talk ...

  6. Fast protein liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/Fast_protein_liquid...

    Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).

  7. Evaporative light scattering detector - Wikipedia

    en.wikipedia.org/wiki/Evaporative_light...

    An evaporative light scattering detector (ELSD) is a destructive chromatography detector, used in conjunction with high-performance liquid chromatography (HPLC), [1] ultra high-performance liquid chromatography (UHPLC), [2] purification liquid chromatography such as flash or preparative chromatography (using a splitter), countercurrent or ...

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