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  2. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Chromatography – a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. Eluent (sometimes spelled eluant) – the solvent or solvent fixure used in elution chromatography and is synonymous with mobile phase.

  3. Affinity chromatography - Wikipedia

    en.wikipedia.org/wiki/Affinity_chromatography

    Affinity chromatography is a method of separating a biomolecule from a mixture, based on a highly specific macromolecular binding interaction between the biomolecule and another substance. The specific type of binding interaction depends on the biomolecule of interest; antigen and antibody, enzyme and substrate, receptor and ligand, or protein ...

  4. Gas chromatography - Wikipedia

    en.wikipedia.org/wiki/Gas_chromatography

    Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture. [1] In preparative chromatography, GC can be ...

  5. Gas chromatography–mass spectrometry - Wikipedia

    en.wikipedia.org/wiki/Gas_chromatography–mass...

    Gas chromatography–mass spectrometry. Gas chromatography–mass spectrometry (GC–MS) is an analytical method that combines the features of gas-chromatography and mass spectrometry to identify different substances within a test sample. [1] Applications of GC–MS include drug detection, fire investigation, environmental analysis, explosives ...

  6. Size-exclusion chromatography - Wikipedia

    en.wikipedia.org/wiki/Size-exclusion_chromatography

    Cytiva, Bio-Rad, Bio-Works, emp Biotech, Knauer, Phenomenex. Size-exclusion chromatography, also known as molecular sieve chromatography, [1] is a chromatographic method in which molecules in solution are separated by their shape, and in some cases size. [2] It is usually applied to large molecules or macromolecular complexes such as proteins ...

  7. Liquid chromatography–mass spectrometry - Wikipedia

    en.wikipedia.org/wiki/Liquid_chromatography...

    Liquid chromatography–mass spectrometry. Liquid chromatography–mass spectrometry (LC–MS) is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry (MS). Coupled chromatography – MS systems are popular in chemical ...

  8. History of chromatography - Wikipedia

    en.wikipedia.org/wiki/History_of_chromatography

    Chromatography, literally "color writing", [1] was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll (which is green) and carotenoids (which are orange and yellow). New forms of chromatography developed in the 1930s and 1940s made the technique useful for a wide range ...

  9. Paper chromatography - Wikipedia

    en.wikipedia.org/wiki/Paper_chromatography

    Paper chromatography is an analytical method used to separate coloured chemicals or substances. [1] It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography (TLC). The setup has three components. The mobile phase is a solution that travels up the ...