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DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.
Flow chart for Hi-C data analysis. [27] Paired-end reads are first iteratively mapped to a reference genome. Mapped reads are then assigned to a restriction fragment/genomic loci, with fragment-level filtering. Data is then binned, filtered at the bin level, and then balanced to correct for potential biases. [27] [28]
The European Nucleotide Archive (ENA) is a repository providing free and unrestricted access to annotated DNA and RNA sequences.It also stores complementary information such as experimental procedures, details of sequence assembly and other metadata related to sequencing projects. [1]
Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
Electroelution is a method used to extract a nucleic acid or a protein sample from an electrophoresis gel by applying a negative current in the plane of the smallest dimension of the gel, drawing the macromolecule to the surface for extraction and subsequent analysis. [2]
FASTQ format is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores.Both the sequence letter and quality score are each encoded with a single ASCII character for brevity.
Rapid DNA is a "swab in-profile out" technology that completely automates the entire DNA extraction, amplification, and analysis process. Rapid DNA instruments are able to go from a swab to a DNA profile in as little as 90 minutes and eliminates the need for trained scientists to perform the process.
The BED (Browser Extensible Data) format is a text file format used to store genomic regions as coordinates and associated annotations.The data are presented in the form of columns separated by spaces or tabs.