Ad
related to: genome vs transcriptome code editor reviews
Search results
Results From The WOW.Com Content Network
RNA-Seq [1] [2] [3] is a technique [4] that allows transcriptome studies (see also Transcriptomics technologies) based on next-generation sequencing technologies. This technique is largely dependent on bioinformatics tools developed to support the different steps of the process.
The term transcriptome is a portmanteau of the words transcript and genome; it is associated with the process of transcript production during the biological process of transcription. The early stages of transcriptome annotations began with cDNA libraries published in the 1980s. Subsequently, the advent of high-throughput technology led to ...
Coverage of genome by assembly: for this metric, BGI's assembly via SOAPdenovo performed best, with 98.8% of the total genome being covered. All assemblers performed relatively well in this category, with all but three groups having coverage of 90% and higher, and the lowest total coverage being 78.5% (Dept. of Comp. Sci., University of Chicago ...
De novo transcriptome assembly is often the preferred method to studying non-model organisms, since it is cheaper and easier than building a genome, and reference-based methods are not possible without an existing genome. The transcriptomes of these organisms can thus reveal novel proteins and their isoforms that are implicated in such unique ...
The information content of an organism is recorded in the DNA of its genome and expressed through transcription. Here, mRNA serves as a transient intermediary molecule in the information network, whilst non-coding RNAs perform additional diverse functions. A transcriptome captures a snapshot in time of the total transcripts present in a cell ...
[136] [137] Limitations of RNA variant identification include that it only reflects expressed regions (in humans, <5% of the genome), could be subject to biases introduced by data processing (e.g., de novo transcriptome assemblies underestimate heterozygosity [138]), and has lower quality when compared to direct DNA sequencing.
Transcriptome vs. genome sequencing [ edit ] Rather than sequencing the entire genome (all DNA sequence) of the various plant species, the project sequenced only those regions of the genome that produce a protein product ( coding genes ); the transcriptome . [ 1 ]
G&T-seq (short for single cell genome and transcriptome sequencing) is a novel form of single cell sequencing technique allowing one to simultaneously obtain both transcriptomic and genomic data from single cells, allowing for direct comparison of gene expression data to its corresponding genomic data in the same cell...