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Release the pipette knob slowly to the ready position. Do not remove the tip from the solution. Repeat this process (step 3 and 4) to rinse the tip until the interior wall of the tip is clear. Remove the tip from the solution. Press the pipette knob to the second stop, and completely empty the tip. Release the pipette knob to the ready position.
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid. Some liquid will remain inside the tip. The liquid remaining in the tip can be dispensed back into the original solution (#4) or thrown away. Release the pipette thumb to the ready ...
Generally, a pipette has optimal accuracy from 35-100% of its nominal volume, and should not be used below 10% of that volume. [5] For example, using a 1,000 μl pipette for 50 μl of liquid is not ideal, and using a 100 μl pipette will give better results. [6] Other factors like tip angle and immersion depth may also impact accuracy ...
For example, a motorized pipette controller can aid liquid aspiration or dispensing using volumetric pipettes or graduated pipettes; [13] a tablet can interact in real-time with the pipette and guide a user through a protocol; [14] and a pipette station can help to control the pipette tip immersion depth and improve ergonomics. [15]
There are two types of pipettes that differ based on where the markings are located in reference to the pipette tip. These are Mohr pipettes and Serological pipettes, and they differ only by the position of the first graduation mark, nearest the tip of the pipette. [3] A Mohr pipette is designed for use as a drain-out pipette. It has a straight ...
(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]