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Both qualities are inversely correlated with resolution: the higher the resolution (i.e. the greater the distance at which two adjacent points can be distinguished as separate), the smaller the depth of field and working distance. Some stereo microscopes can deliver a useful magnification up to 100×, comparable to a 10× objective and 10× ...
In certain applications, long-working-distance or long-focus microscopes [29] are beneficial. An item may need to be examined behind a window, or industrial subjects may be a hazard to the objective. Such optics resemble telescopes with close-focus capabilities. [30] [31] Measuring microscopes are used for precision measurement.
A USB microscope is a low-powered digital microscope which connects to a ... The precise magnification is determined by the working distance between the camera and ...
In microscopy the Barlow lens is used to increase working distance and decrease magnification. The lenses are "objective lenses" that are mounted in front of the microscope's last objective element. Barlow lenses for microscopes can be found with magnifications ranging from 0.3× to 2×.
Some scanning tunneling microscopes are capable of recording images at high frame rates. [11] [12] Videos made of such images can show surface diffusion [13] or track adsorption and reactions on the surface. In video-rate microscopes, frame rates of 80 Hz have been achieved with fully working feedback that adjusts the height of the tip. [14]
Oil immersion objectives can have practical difficulties due to their shallow depth of field and extremely short working distance, which calls for the use of very thin (0.17 mm) cover slips, or, in an inverted microscope, thin glass-bottomed Petri dishes.
The near-field optical (NFO) microscope involved a sub-wavelength aperture at the apex of a metal coated sharply pointed transparent tip, and a feedback mechanism to maintain a constant distance of a few nanometers between the sample and the probe. Lewis et al. were also aware of the potential of an NFO microscope at this time. [14]
SPDM (spectral precision distance microscopy) is a family of techniques in fluorescence microscopy which gets around this problem by measuring just a few sources at a time, so that each source is "optically isolated" from the others (i.e., separated by more than the microscope's resolution, typically ~200-250 nm).