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The term in situ in the medical context is part of a group of two-word Latin expressions, including in vitro, in vivo, and ex vivo. Similar to abbreviations, these terms support the concise transfer of essential information in medical communication. In situ is among the most widely used and versatile Latin terms in medical discourse in modern ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. RNA probes can be designed for any gene or any sequence within a gene for visualization of mRNA , [ 3 ] [ 4 ] [ 5 ] lncRNA [ 6 ] [ 7 ] [ 8 ] and miRNA in tissues and cells.
In-situ conservation is the on-site conservation or the conservation of genetic resources in natural populations of plant or animal species, such as forest genetic resources in natural populations of tree species. [1] This process protects the inhabitants and ensures the sustainability of the environment and ecosystem.
Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact tissue. [1] The historical precursor to spatial transcriptomics is in situ hybridization, [2] where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets.
Chromogenic in situ hybridization (CISH) is a cytogenetic technique that combines the chromogenic signal detection method of immunohistochemistry (IHC) techniques with in situ hybridization. [ 1 ] [ 2 ] It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene ...
The resolution that can be achieved with in situ Hi-C can be up to 950 to 1000 bp compared to the 1 to 10 Mb resolution of standard Hi-C and the 100 kb resolution of DNase Hi-C. [3] [4] [14] [19] While standard Hi-C makes use of a 6-bp cutter such as HindIII for the restriction digest step, in situ Hi-C uses a 4-bp cutter such as MboI or its ...
This list of research methods in biology is an index to articles ... Used of an antibody-based method to detect a specific protein in a sample: Molecular biology, ...