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The isotropic expansion of the emitted loop which, by decreasing the back-stress on the source like the inverse of the loop radius, will allow the reactivation of emission events. Both steps depend on the pair interactions and line tension. As such, they are liable to hydrogen effects.
In physics and geometry, isotropy (from Ancient Greek ἴσος (ísos) 'equal' and τρόπος (trópos) 'turn, way') is uniformity in all orientations. Precise definitions depend on the subject area. Exceptions, or inequalities, are frequently indicated by the prefix a- or an-, hence anisotropy.
Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout...
ZOOM, a new tissue expansion technique based on the hydrogel conversion reaction, is developed to enable scalable and isotropic expansion of biological samples with easily tunable expansion ratio (up to eightfold).
Nature Methods - 20ExM achieves isotropic ~20× expansion of cells and tissues in a single shot for super-resolution imaging with <20-nm resolution on a conventional microscope.
This method allows for simple and flexible expansion of a wide range of biological samples, from bacteria to human brain tissues, for super‐resolution imaging of samples with ordinary microscopes.
Here we mathematically present that, even if the expansion is isotropic, tissues located on the embryos are asymmetrically deformed by the expansion, resulting in elongation. We generalize the effect of expanding environments on tissue elongation through model reduction and uncover the mechanism underlying elongation.
One-step nanoscale expansion (ONE) microscopy enables the visualization of the shapes of individual membrane and soluble proteins, achieving around 1-nm resolution. We show that conformational ...
Careful controls for isotropic expansion in all spatial dimensions are essential for reliable results. Here, we summarize the progress made so far in improving the ExM technique, applying it to different systems, ensuring sufficient labeling and accurate data analysis, and transferring it to novel applications.
We established the mechanism underlying tissue elongation induced by isotropically expanding external environments, and its involvement in collective cell alignment with cell elongation, providing key insight into morphogenesis involving multiple adjacent tissues.