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In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction.It terminates any ongoing biochemical reactions and may also increase the treated tissues' mechanical strength or stability.
Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for the electron microscopy and cryo-electron microscopy. [1] Typical specimens for cryofixation include small samples of plant or animal tissue , cell suspensions of microorganisms or cultured cells , suspensions of ...
Histologic specimen being placed on the stage of an optical microscope. Human lung tissue stained with hematoxylin and eosin as seen under a microscope.. Histology, [help 1] also known as microscopic anatomy or microanatomy, [1] is the branch of biology that studies the microscopic anatomy of biological tissues.
Microtechnique is an aggregate of methods used to prepare micro-objects for studying. [1] It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique.
Zenker's fixative is a rapid-acting fixative for animal tissues. It is employed to prepare specimens of animal or vegetable tissues for microscopic study. It provides excellent fixation of nuclear chromatin, connective tissue fibers and some cytoplasmic features, but does not preserve delicate cytoplasmic organelles such as mitochondria.
Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they do not rinse out during the staining procedure. The addition of iodine, which binds to crystal violet and traps it in the cell; Rapid decolorization with ethanol or acetone; Counterstaining with safranin. [13]
Bouin's fluid is especially useful for fixation of gastrointestinal tract biopsies because this fixative allows crisper and better nuclear staining than 10% neutral-buffered formalin. It is not a good fixative when tissue ultrastructure must be preserved for electron microscopy. However, it is a good fixative when tissue structure with a soft ...
The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...