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In enzymology, a sucrose synthase (EC 2.4.1.13) is an enzyme that catalyzes the chemical reaction NDP-glucose + D-fructose ⇌ NDP + sucrose Thus, the two substrates of this enzyme are NDP-glucose and D-fructose , whereas its two products are NDP and sucrose .
Sucrose-phosphate synthase (SPS) is a plant enzyme involved in sucrose biosynthesis. Specifically, this enzyme catalyzes the transfer of a hexosyl group from uridine diphosphate glucose ( UDP-glucose ) to D- fructose 6-phosphate to form UDP and D-sucrose-6-phosphate.
Sucrose is formed by plants, algae and cyanobacteria but not by other organisms. Sucrose is the end product of photosynthesis and is found naturally in many food plants along with the monosaccharide fructose. In many fruits, such as pineapple and apricot, sucrose is the main sugar. In others, such as grapes and pears, fructose is the main sugar.
In plants, sucrose transport is distributed throughout the plant by the proton-pump where the pump creates a gradient of protons so that there are many more on one side of the membrane than the other. As the protons diffuse back across the membrane, the free energy liberated by this diffusion is used to co-transport sucrose. In mammals, glucose ...
Phloem (/ ˈ f l oʊ. əm /, FLOH-əm) is the living tissue in vascular plants that transports the soluble organic compounds made during photosynthesis and known as photosynthates, in particular the sugar sucrose, [1] to the rest of the plant. This transport process is called translocation. [2]
In biochemistry, a metabolic pathway is a linked series of chemical reactions occurring within a cell.The reactants, products, and intermediates of an enzymatic reaction are known as metabolites, which are modified by a sequence of chemical reactions catalyzed by enzymes.
Sucrose phosphorylase (EC 2.4.1.7) is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases . More specifically it has been placed in the retaining glycoside hydrolases family although it catalyzes a transglycosidation rather than hydrolysis.
Sucrose is a non-reducing sugar, so will not test positive with Benedict's solution. To test for sucrose, the sample is treated with sucrase. The sucrose is hydrolysed into glucose and fructose, with glucose being a reducing sugar, which in turn tests positive with Benedict's solution. [citation needed].