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Fluorescence in several wavelengths can be detected by an array detector, to detect compounds from HPLC flow. Also, TLC plates can be visualized if the compounds or a coloring reagent is fluorescent. Fluorescence is most effective when there is a larger ratio of atoms at lower energy levels in a Boltzmann distribution. There is, then, a higher ...
Chemiluminescence is different from fluorescence. Hence, fluorescent proteins such as Green fluorescent protein are not chemiluminescent. However, combining GFP with luciferases allows bioluminescence resonance energy transfer (BRET), which increases the quantum yield of light emitted in these systems.
A fluorescent lamp, or fluorescent tube, is a low-pressure mercury-vapor gas-discharge lamp that uses fluorescence to produce visible light. An electric current in the gas excites mercury vapor, to produce ultraviolet and make a phosphor coating in the lamp glow.
In beam penetration tubes, different color phosphors are layered and separated with dielectric material. The acceleration voltage is used to determine the energy of the electrons; lower-energy ones are absorbed in the top layer of the phosphor, while some of the higher-energy ones shoot through and are absorbed in the lower layer.
Transition metal compounds are often colored because of transitions of electrons between d-orbitals of different energy. (see Transition metal#Colored compounds ). Organic compounds tend to be colored when there is extensive conjugation , causing the energy gap between the HOMO and LUMO to decrease, bringing the absorption band from the UV to ...
Different color glow sticks meant for use as bracelets. A glow stick, also known as a light stick, chem light, light wand, light rod, and rave light, is a self-contained, short-term light-source. It consists of a translucent plastic tube containing isolated substances that, when combined, make light through chemiluminescence. [1]
Atomic Fluorescence Spectroscopy (AFS) techniques are useful in other kinds of analysis/measurement of a compound present in air or water, or other media, such as CVAFS which is used for heavy metals detection, such as mercury. Fluorescence can also be used to redirect photons, see fluorescent solar collector.
Radioluminescent 1.8-curie (67 GBq) 6-by-0.2-inch (152.4 mm × 5.1 mm) tritium vials are tritium gas-filled, thin glass vials with inner surfaces coated with a phosphor. Tritium radioluminescence is the use of gaseous tritium , a radioactive isotope of hydrogen , to create visible light.