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Fluorescence is the phenomenon of absorption of electromagnetic radiation, typically from ultraviolet or visible light, by a molecule and the subsequent emission of a photon of a lower energy (smaller frequency, longer wavelength). This causes the light that is emitted to be a different color than the light that is absorbed.
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
Micrograph of paper autofluorescing under ultraviolet illumination. The individual fibres in this sample are around 10 μm in diameter.. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores).
Iridescence in soap bubbles. Iridescence (also known as goniochromism) is the phenomenon of certain surfaces that appear gradually to change colour as the angle of view or the angle of illumination changes.
Fluorescence of different substances under UV light. Green is a fluorescein, red is Rhodamine B, yellow is Rhodamine 6G, blue is quinine, purple is a mixture of quinine and rhodamine 6g. Solutions are about 0.001% concentration in water. Fluorophore molecules could be either utilized alone, or serve as a fluorescent motif of a functional system.
Color CRTs require three different phosphors, emitting in red, green and blue, patterned on the screen. Three separate electron guns are used for color production (except for displays that use beam-index tube technology, which is rare). The red phosphor has always been a problem, being the dimmest of the three necessitating the brighter green ...
These characters may produce a variety of colors depending on the pigment responsible for fluorescence. [ 10 ] [ 8 ] [ 5 ] [ 9 ] While it is unclear what the primary function of different kinds of fluorescence are in plants, [ 4 ] reproductive characters may biofluoresce as a signal to attract pollinators, [ 11 ] [ 9 ] However, biofluorescence ...
The fluorescent chemicals in fluorescent paint absorb the invisible UV radiation, then emit the energy as longer wavelength visible light of a particular color. Human eyes perceive this light as the unusual 'glow' of fluorescence. The painted surface also reflects any ordinary visible light striking it, which tends to wash out the dim ...