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BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...
Bicinchoninic acid (/ b aɪ s ɪ n k ɔː n ɪ n ɪ k /) or BCA is a weak acid composed of two carboxylated quinoline rings. It is an organic compound with the formula (C 9 H 5 NCO 2 H) 2 . The molecule consists of a pair of quinoline rings, each bearing a carboxylic acid group.
In 1947, the BCA Crime Lab was established in St. Paul to assist in solving of crimes via forensic science, and was one of the first DNA laboratories in the United States in 1990. [6] Later the BCA was the first law enforcement agency in the United States to identify a suspect solely on DNA.
The most common reasons for laboratory semen analysis in humans are as part of a couple's infertility investigation and after a vasectomy to verify that the procedure was successful. [4] It is also commonly used for testing human donors for sperm donation , and for animals semen analysis is commonly used in stud farming and farm animal breeding .
Lately, the Zeiss-SMT VP-SEM has been extended to higher pressure together with a gaseous ionization or gaseous scintillation as the SE mechanism for image formation. Therefore, it is improper to identify the term ESEM with one only brand of commercial instrument in juxtaposition to other competing commercial (or laboratory) brands with ...
An account of the early history of scanning electron microscopy has been presented by McMullan. [2] [3] Although Max Knoll produced a photo with a 50 mm object-field-width showing channeling contrast by the use of an electron beam scanner, [4] it was Manfred von Ardenne who in 1937 invented [5] a microscope with high resolution by scanning a very small raster with a demagnified and finely ...
Essential features of a biosafety level 4 (BSL-4) laboratory [1]. A biosafety level (BSL), or pathogen/protection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility.
RELAP5-3D is an outgrowth of the one-dimensional RELAP5/MOD3 code developed at Idaho National Laboratory (INL) for the U.S. Nuclear Regulatory Commission (NRC). The U.S. Department of Energy (DOE) began sponsoring additional RELAP5 development in the early 1980s to meet its own reactor safety assessment needs.