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The test uses the principles of gel electrophoresis to separate out the various types of hemoglobin and is a type of native gel electrophoresis.After the sample has been treated to release the hemoglobin from the red cells, it is introduced into a porous gel (usually made of agarose or cellulose acetate) and subjected to an electrical field, most commonly in an alkaline medium.
Schematic diagram of 3-part analyzer. A 3-part differential cell counter uses Coulter's principle to find the size and volume of the cell. The sample is lysed and dissolved into an electrolyte solution in a container, which also holds a smaller container.
Protein electrophoresis (general technique—not a specific test) Western blot (general technique—not a specific test) Liver function tests; Polymerase chain reaction (DNA). DNA profiling is today possible with even very small quantities of blood: this is commonly used in forensic science, but is now also part of the diagnostic process of ...
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Electroelution is a method used to extract a nucleic acid or a protein sample from an electrophoresis gel by applying a negative current in the plane of the smallest dimension of the gel, drawing the macromolecule to the surface for extraction and subsequent analysis. [2]
These components comprise hemoglobin and others. [3] At this stage, the hemoglobin is called free hemoglobin. [3] Free hemoglobin (also called naked hemoglobin) is the un-bound hemoglobin that is not enclosed in the red blood cell. The naked hemoglobin is devoid of its anti-oxidant sentries that are normally available within the RBC. [5]
Diagram of continuous flow spectrometer for reactions with half times of a few milliseconds. The stopped-flow method evolved from the continuous-flow technique developed by Hamilton Hartridge and Francis Roughton [7] to study the binding of oxygen to hemoglobin. In the continuous-flow system, the reaction mixture was passed through a long tube ...