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PCT is a patented, enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels (up to 90,000 psi) to safely, conveniently and reproducibly control the actions of molecules in biological samples, e.g., the rupture (lysis) of cells and tissues from human, animal, plant, and microbial ...
The French pressure cell press, or French press, is an apparatus used in biological experimentation to disrupt the plasma membrane of cells by passing them through a narrow valve under high pressure. [1] The French press can also be used for disintegration of chloroplasts, homogenates of animal tissue, and other biological particles.
Homogenization of tissue in solution is often performed simultaneously with cell lysis. To prevent lysis however, the tissue (or collection of cells, e.g. from cell culture) can be kept at temperatures slightly above zero to prevent autolysis, and in an isotonic solution to prevent osmotic damage. [2]
A homogenizer is a piece of laboratory or industrial equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Many different models have been developed using various physical technologies for disruption.
Cell lysis is used in laboratories to break open cells and purify or further study their contents. Lysis in the laboratory may be affected by enzymes or detergents or other chaotropic agents . Mechanical disruption of cell membranes, as by repeated freezing and thawing, sonication , pressure, or filtration may also be referred to as lysis.
Milk homogenization is accomplished by mixing large amounts of harvested milk, then forcing the milk at high pressure through small holes. [7] Milk homogenization is an essential tool of the milk food industry to produce consistent levels of flavor and fat concentration. Another application of homogenization is in soft drinks like cola products.
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