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Chromatographic peak resolution is given by = + where t R is the retention time and w b is the peak width at baseline. The bigger the time-difference and/or the smaller the bandwidths, the better the resolution of the compounds.
The variance per unit length of the column is taken as the ratio of the column length to the column efficiency in theoretical plates. The van Deemter equation is a hyperbolic function that predicts that there is an optimum velocity at which there will be the minimum variance per unit column length and, thence, a maximum efficiency. The van ...
An R F value will always be in the range 0 to 1; if the substance moves, it can only move in the direction of the solvent flow, and cannot move faster than the solvent. For example, if particular substance in an unknown mixture travels 2.5 cm and the solvent front travels 5.0 cm, the retardation factor would be 0.50.
It can be computed by ... For the case of column vector c and row vector r, ... where ,, are column vectors of length 3, then the gradient over one of the ...
The length of the column is very large as compared to the cross-sectional dimensions of the column. The column fails only by buckling. This is true if the compressive stress in the column does not exceed the yield strength σ y {\displaystyle \sigma _{y}} (see figure 1): σ = P c r A = π 2 E ( L e / r ) 2 < σ y {\displaystyle \sigma ={\frac ...
A size exclusion column. Each size exclusion column has a range of molecular weights that can be separated. The exclusion limit defines the molecular weight at the upper end of the column 'working' range and is where molecules are too large to get trapped in the stationary phase.
The column vectors of a matrix. The column space of this matrix is the vector space spanned by the column vectors. ... { r 1, r 2, r 3, r 4}. Since these four row ...
The slenderness ratio is an indicator of the specimen's resistance to bending and buckling, due to its length and cross section. If the slenderness ratio is less than the critical slenderness ratio, the column is considered to be a short column. In these cases, the Johnson parabola is more applicable than the Euler formula. [5]