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  2. dnaB helicase - Wikipedia

    en.wikipedia.org/wiki/DnaB_helicase

    DnaB helicase is an enzyme in bacteria which opens the replication fork during DNA replication.Although the mechanism by which DnaB both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown, a change in the quaternary structure of the protein involving dimerisation of the N-terminal domain has been observed and may occur during the enzymatic cycle. [1]

  3. DnaA - Wikipedia

    en.wikipedia.org/wiki/DnaA

    DnaC helps the helicase to bind to and to properly accommodate the ssDNA at the 13 bp region; this is accomplished by ATP hydrolysis, after which DnaC is released. Single-strand binding proteins (SSBs) stabilize the single DNA strands in order to maintain the replication bubble. DnaB is a 5'→3' helicase, so it travels on the lagging strand.

  4. Replisome - Wikipedia

    en.wikipedia.org/wiki/Replisome

    Helicase is an enzyme which breaks hydrogen bonds between the base pairs in the middle of the DNA duplex. Its doughnut like structure wraps around DNA and separates the strands ahead of DNA synthesis. In eukaryotes, the Mcm2-7 complex acts as a helicase, though which subunits are required for helicase activity is not entirely clear. [2]

  5. Primosome - Wikipedia

    en.wikipedia.org/wiki/Primosome

    In Bacillus subtilis, genetic analysis has revealed three primosomal proteins, DnaB, DnaD, and DnaI, that have no obvious homologues in E. coli. They are involved in primosome function both at arrested replication forks and at the chromosomal origin. Our biochemical analysis of the DnaB and DnaD proteins unravels their role in primosome assembly.

  6. Pre-replication complex - Wikipedia

    en.wikipedia.org/wiki/Pre-replication_complex

    In prokaryotes, DnaA hydrolyzes ATP in order to unwind DNA at the oriC. This denatured region is accessible to the DnaB helicase and DnaC helicase loader. Single-strand binding proteins stabilize the newly formed replication bubble and interact with the DnaG primase. DnaG recruits the replicative DNA polymerase III, and replication begins.

  7. DnaB - Wikipedia

    en.wikipedia.org/wiki/DnaB

    Printable version; In other projects Wikidata item; Appearance. move to sidebar hide. DnaB may refer to: DNA helicase, an enzyme class; dnaB helicase, a bacterial ...

  8. DNA unwinding element - Wikipedia

    en.wikipedia.org/wiki/DNA_unwinding_element

    The DNA helicase and associated enzymes are now able to bind to the unwound region, creating a replication fork start. The unwinding of this duplex strand region is associated with a low free energy requirement, due to helical instability caused by specific base-stacking interactions, in combination with counteracting supercoiling.

  9. DNA-dependent ATPase - Wikipedia

    en.wikipedia.org/wiki/DNA-dependent_ATPase

    DNA-dependent ATPase, abbreviated Dda and also known as Dda helicase and Dda DNA helicase, is the 439-amino acid 49,897-atomic mass unit protein coded by the Dda gene of the bacteriophage T4 phage, a virus that infects enterobacteria.

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