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One of the fundamentals of flow cytometry is the ability to measure the properties of individual particles. When a sample enters a flow cytometer, the particles are randomly distributed in the 3-D space of the sample line, the diameter of which is significantly larger than the diameter of most cells.
Flow cytometry is a cell analysis technique that was first used in the 1950s to measure the volume of cells in a rapidly flowing fluid stream as they passed in front of a viewing aperture.
In this guide, we’ll take you through flow cytometry basics, principles, protocols, and analysis to give you an advanced understanding of how flow cytometry works, when it is valuable, and how to do it. You will also find everything you need to quickly and easily set up your multicolor flow cytometry experiment, from
Flow cytometry rapidly analyzes physical and chemical properties of cells and particles. Here’s how it works: A lab technician places a sample of your blood , tissue or bone marrow in a suspension (liquid), treats the cell suspension with fluorescently tagged antibodies , and injects it into the flow cytometer.
This article provides a concise guide to the principles and utilization of flow cytometry. Step-by-step instructions, from instrument setup to data analysis, are intended to help researchers successfully apply flow cytometry in their studies. Previous. Next.
Flow cytometry is a laboratory technique that is used to analyze cells. Reviewing the basics of flow cytometry, immunodetection methods, key flow cytometry protocol steps, and proper controls helps to ensure accurate flow cytometric analysis. Read on to uncover tips that will make your next flow cytometry experiment successful.
Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity.
The basic principle of flow cytometry is based on the measurement of light scattered by particles, and the fluorescence observed when these particles are passed in a stream through a laser beam. Figure: Schematic of a common flow cytometer, illustrating the fluidic, optical, and electronic systems. Image Source: AAT Bioquest, Inc. Light Scattering.
Learn about how flow cytometers work and get information to help you get started with your flow cytometry experiments.
button-secondary. icon-none. What is flow cytometry? Flow cytometry is a widely used, laser-based method for analyzing the expression of cell surface and intracellular molecules.