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Protein purification is a fundamental step for analyzing individual proteins and protein complexes and identifying interactions with other proteins, DNA or RNA. A variety of protein purification strategies exist to address desired scale, throughput and downstream applications.
This guide provides a comprehensive introduction to DNA and RNA purification methods, including the basics of DNA isolation, plasmid growth and nucleic acid quantification.
Affinity tags are a selective and versatile way to purify your protein of interest. Use this introductory guide to learn about what tagging systems are available and how they work.
Whether you need an affinity tag for protein purification, want to create a single fusion protein to be used in a variety of protein analysis methods, or add an endogenous tag using CRISPR-Cas9 gene editing, there are an array of tags and reporter enzymes available.
Promega’s unique portfolio of protein analysis laboratory products include mass spec proteases, protein purification tags/resins and fluorescent ligands, cell-free and cell-based protein expression systems, and novel methods for detecting and studying proteins at endogenous expression levels.
Whether you need an affinity tag for protein purification, want to create a single fusion protein to be used in a variety of protein analysis methods, or add an endogenous tag using CRISPR-Cas9 gene editing, there are an array of tags and reporter enzymes available.
The HaloTag® Protein Purification System(a–d) is based on a unique tag, the HaloTag® protein, which allows covalent, eficient and specific capture of the proteins expressed in E. coli as N-terminal HaloTag® fusion proteins.
HaloTag® Protein Purification Systems provide a rapid, reliable method for purification of proteins expressed in E. coli and mammalian cells. For E. coli, the HaloTag® protein tag enhances expression and solubility of recombinant proteins.
In this study, we show that protein purification using the HaloTag® protein tag offers significant advantage over 3x FLAG® and His6tag methods with respect to yield and purity.
The Magne™ HaloTag ® Beads allow protein purification through covalent capture of HaloTag ® fusion proteins and subsequent release of these target proteins by TEV protease cleavage without multiple centrifugation steps, providing a simple purification that can be performed on robotic platforms.