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Good's buffers (also Good buffers) are twenty buffering agents for biochemical and biological research selected and described by Norman Good and colleagues during 1966–1980. [ 1 ] [ 2 ] [ 3 ] Most of the buffers were new zwitterionic compounds prepared and tested by Good and coworkers for the first time, though some ( MES , ADA , BES , Bicine ...
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MOPSO is a zwitterionic organic chemical buffering agent; one of Good's buffers. MOPSO and MOPS (3-morpholinopropanesulfonic acid) are chemically similar, differing only in the presence of a hydroxyl group on the C-2 of the propane moiety. It has a useful pH range of 6.5-7.9 in the physiological range, making it useful for cell culture work.
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MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES, [1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring.
In general, as temperature decreases from 25 °C to 5 °C the pH of a tris buffer will increase an average of 0.03 units per degree. As temperature rises from 25 °C to 37 °C, the pH of a tris buffer will decrease an average of 0.025 units per degree.
Even though size exclusion chromatography is widely utilized to study natural organic material, there are limitations. One of these limitations include that there is no standard molecular weight marker; [6] thus, there is nothing to compare the results back to. If precise molecular weight is required, other methods should be used.
Total ionic strength adjustment buffer (TISAB) is a buffer solution which increases the ionic strength of a solution to a relatively high level. This is important for potentiometric measurements, including ion selective electrodes , because they measure the activity of the analyte rather than its concentration.