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Multi-wavelength anomalous diffraction (sometimes Multi-wavelength anomalous dispersion; abbreviated MAD) is a technique used in X-ray crystallography that facilitates the determination of the three-dimensional structure of biological macromolecules (e.g. DNA, drug receptors) via solution of the phase problem.
A powerful solution is the multi-wavelength anomalous dispersion (MAD) method. In this technique, atoms' inner electrons [clarification needed] absorb X-rays of particular wavelengths, and reemit the X-rays after a delay, inducing a phase shift in all of the reflections, known as the anomalous dispersion effect. Analysis of this phase shift ...
When combined with basic optical spectrometers such as prisms or diffraction gratings and automated, ground-based observation platforms, it presents a cheap and powerful means for the measurement of trace gas species such as ozone and nitrogen dioxide.
As in multi-wavelength anomalous dispersion phasing, the changes in the scattering amplitudes can be interpreted to yield the phases. Although this is the original method by which protein crystal structures were solved, it has largely been superseded by multi-wavelength anomalous dispersion phasing with selenomethionine.
Anomalous X-ray scattering (AXRS or XRAS) is a non-destructive determination technique within X-ray diffraction that makes use of the anomalous dispersion that occurs when a wavelength is selected that is in the vicinity of an absorption edge of one of the constituent elements of the sample. It is used in materials research to study nanometer ...
Holographic interferometry is a technique which uses holography to monitor small deformations in single wavelength implementations. In multi-wavelength implementations, it is used to perform dimensional metrology of large parts and assemblies and to detect larger surface defects. [2]: 111–120
Spectroscopy is a branch of science concerned with the spectra of electromagnetic radiation as a function of its wavelength or frequency measured by spectrographic equipment, and other techniques, in order to obtain information concerning the structure and properties of matter. [4]
Diffraction is the same physical effect as interference, but interference is typically applied to superposition of a few waves and the term diffraction is used when many waves are superposed. [1]: 433 Italian scientist Francesco Maria Grimaldi coined the word diffraction and was the first to record accurate observations of the phenomenon in 1660.