Search results
Results From The WOW.Com Content Network
Disassociate the RNA from the protein of interest. Isolate the RNA fragments from the protein using a centrifuge. Use Reverse Transcription PCR to convert the RNA fragments into cDNA (DNA that is complementary to the RNA fragments). Fluorescently label these cDNA fragments. Prepare the gene chip. This is a small chip that has DNA sequences ...
RNA partitions in the aqueous phase, while proteins and DNA partition into the organic/interphase (left). The RNA is then precipitated in an alcohol (right). Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry and molecular biology.
Immunoprecipitation of intact protein complexes (i.e. antigen along with any proteins or ligands that are bound to it) is known as co-immunoprecipitation (Co-IP). Co-IP works by selecting an antibody that targets a known protein that is believed to be a member of a larger complex of proteins.
Jill recently shared a cooking hack video that includes a fun and easy science project for kids that can be done in your own kitchen!. In the video, Jill starts by grabbing a glass of water and a ...
For premium support please call: 800-290-4726 more ways to reach us
Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to identify RNA binding sites of proteins on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks.
Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA, and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding salt and ethanol as an antisolvent. In DNA extraction, after separating DNA from other cell constituents in water, DNA is precipitated out of solution by neutralizing it with positively ...
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) [1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.