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Brain heart infusion media made from Difco powdered media. Brain heart infusion (BHI) is a growth medium for growing microorganisms. It is a nutrient-rich medium, and can therefore be used to culture a variety of fastidious organisms.
Rappaport-Vassiliadis soya peptone broth (RVS broth) is used as an enrichment growth medium for the isolation of Salmonella species. [1] It is not recommended for the enrichment of Salmonella Typhi or Paratyphi, which is inhibited due to the malachite green in RVS broth. [2] It is an alternative to selenite broth.
The standard can be compared visually to a suspension of bacteria in sterile saline or nutrient broth. If the bacterial suspension is too turbid, it can be diluted with more diluent. If the suspension is not turbid enough, more bacteria can be added. McFarland nephelometer standards:{2}
Thioglycolate broth is a multipurpose, enrichment, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes . [ 1 ]
This pepsic peptone was developed by DIFCO (Digestive Ferments Company) during the First World War for producing bacterial toxins for vaccine production. [ 15 ] Fort development of red-brick colonies of GBS in granada medium it is necessary the presence of the peptide Ile-Ala-Arg-Arg-His-Pro-Tyr-Phe in the culture medium.
Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani , intended LB to stand for lysogeny broth , [ 1 ] but LB has also come to colloquially mean Luria broth , Lennox broth , life broth or Luria –Bertani medium . [ 2 ]
The composition of Mueller Hinton agar can affect the growth characteristics of bacterial isolates, as well as their response to antibiotics. For example, variations in the pH of the medium can affect the activity of certain antibiotics, and the presence of certain nutrients can promote the growth of specific bacterial species.
Nutrient broth has the same composition, but lacks agar. [ 2 ] [ 3 ] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.