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The API 20E/NE system is a widely used biochemical test system designed for the rapid identification of Gram-negative bacteria. It specifically targets two groups: Enterobacteriaceae (API 20E) and non-Enterobacteriaceae (API 20NE).
When P. vulgaris is tested using the API 20E identification system [1] it produces positive results for sulfur reduction, urease production, tryptophan deaminase production, indole production, sometimes positive gelatinase activity, and saccharose fermentation, and negative results for the remainder of the tests on the testing strip.
Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...
Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.
Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3]
McFarland standards. No. 0.5, 1 and 2. In microbiology, McFarland standards are used as a reference to adjust the turbidity of bacterial suspensions so that the number of bacteria will be within a given range to standardize microbial testing.
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