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These labeled cell suspension is then put into a separation column in a strong magnetic field. The labeled cells are contained, magnetized, while in the magnetic field and the unlabeled cells are suspended, un-magnetized, to be collected. Once removed from magnetic field positive cells are eluted.
3D cell culture methods have been developed to enable research into the behavior of cells in an environment that represents their interactions in-vivo more accurately [5].. 3D cell culturing by magnetic levitation uses biocompatible polymer-based reagents [2] to deliver magnetic nanoparticles to individual cells, so that an applied magnetic driver can levitate cells off the bottom of the cell ...
Human cells have also been manipulated in Digital Microfluidic Immunocytochemistry in Single Cells (DISC) where DMF platforms were used to culture and use antibodies to label phosphorylated proteins in the cell. [86] Cultured cells are then removed and taken off chip for screening. Another technique synthesizes hydrogels within DMF platforms.
A magnetic-labelled receptor that binds to annexin is added to sperm. Inside normal cells, phosphatidylserine molecules are located within the cell membrane towards the cytoplasm. Nevertheless, in those cells that initiate the apoptotic process phosphatidylserine instead faces the cell membrane outer side, binding to the annexin conjugate.
A pinched aluminium can, produced from a pulsed magnetic field created by rapidly discharging 2 kilojoules from a high-voltage capacitor bank into a 3-turn coil of heavy gauge wire. Electromagnetic forming (EM forming or magneforming) is a type of high-velocity, cold forming process for electrically conductive metals, most commonly copper and ...
A handheld microfluidic cell culture incubator capable of heating and pumping cell culture solutions has also been developed. [46] Due to the volume reduction in microfluidic cultures, the collected concentrations are higher for better signal-to-noise ratio measurements, but collection and detection is correspondingly more difficult. [ 3 ]
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Pulsed-power water treatment is the process of using pulsed, electro-magnetic fields into cooling water to control scaling, biological growth, and corrosion. The process does not require the use of chemicals and helps eliminate environmental and health issues associated with the use and life-cycle management of chemicals used to treat water. [ 1 ]