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Principle of Citrate Utilization Test. Citrate agar is used to test an organism’s ability to utilize citrate as a source of energy. The medium contains citrate as the sole carbon source and inorganic ammonium salts (NH4H2PO4) as the sole source of nitrogen.
The citrate utilization test is a part of the IMViC test (Indole, Methyl Red, Vogues-Proskauer, and Citrate Test) that differentiates organisms on the basis of their ability to use citrate as a sole source of energy.
The citrate test is often part of a battery of tests used to identify gram-negative pathogens and environmental isolates (10). For instance, test kits such as the API-20E (bioMerieux) and Enterotube II (BD Diagnostics) include citrate utilization medium as one of the diagnostic tests.
A citrate utilization test is used to determine the ability of bacteria to utilize sodium citrate as its only carbon source and inorganic ammonium dihydrogen phosphate (NH4H2PO4) as the sole nitrogen source.
Explain what the citrate test indicates about a bacteria species' genes, enzymes, and metabolism. Tell what citrate permease is and what it does for bacterial species that have it. Tell that the citrate test is useful for bacterial identification and characterization.
The citrate test detects the ability of an organism to use citrate as the sole source of carbon and energy. Principle. Bacteria are inoculated on a medium containing sodium citrate and a pH indicator such as bromothymol blue. The medium also contains inorganic ammonium salts, which are utilized as sole source of nitrogen.
Citrate utilization is a IMViC test procedure that discriminates the different genera of Enterobacteriaceae family based on citrate utilization by an enzyme catalyst (citrase) produced by the test organism.
A citrate utilization test helps detect the organism’s ability to produce citrase enzyme. Such organisms can use sodium citrate as a source of carbon as well as inorganic ammonium salt as the source of nitrogen.
The citrate test screens a bacterial isolate for the ability to utilize citrate as its carbon and energy source. A positive diagnostic test rests on the generation of alkaline by-products of citrate metabolism.
What is Citrate Utilization Test? Citrate Utilization Test is a biochemical test in the IMViC test series which detects the ability of organisms (bacteria) to utilize citrate as a sole source of energy.