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This design is very different from that of Sanger sequencing—also known as capillary sequencing or first-generation sequencing—which is based on electrophoretic separation of chain-termination products produced in individual sequencing reactions. [6] This methodology allows sequencing to be completed on a larger scale. [7]
This sequencing method is based on reversible dye-terminators that enable the identification of single nucleotides as they are washed over DNA strands. It can also be used for whole- genome and region sequencing, transcriptome analysis, metagenomics , small RNA discovery, methylation profiling, and genome-wide protein - nucleic acid interaction ...
Illumina produces a number of next-generation sequencing machines using technology acquired from Manteia Predictive Medicine and developed by Solexa. [19] Illumina makes a number of next generation sequencing machines using this technology including the HiSeq, Genome Analyzer IIx, MiSeq and the HiScanSQ, which can also process microarrays. [20]
Illumina Announces Sequencing Technology Innovations Rich Pipeline Delivers the Most Comprehensive End-to-End Solutions for Next-Generation Sequencing SAN DIEGO--(BUSINESS WIRE)-- Illumina (NAS ...
Klenerman, along with Shankar Balasubramanian, invented a method of next-generation DNA sequencing which is commonly known today as the Solexa sequencing or Illumina dye sequencing. [5] [7] The method is based on the detection of fluorophore labelled nucleotides as they get incorporated in the DNA strands. [16]
Illumina uses next-generation sequencing, which is far faster and more efficient than traditional Sanger sequencing. [57] Illumina sequencers perform short-read sequencing, and are image based, utilizing Illumina dye sequencing. [57] This technology has a higher accuracy than long-read sequencing. [57]
ClickSeq is a click-chemistry based method for generating next generation sequencing libraries for deep-sequencing platforms including Illumina, HiSeq, MiSeq and NextSeq. [1] [2] [3] Its function is similar to most other techniques for generating RNAseq or DNAseq libraries in that it aims to generate random fragments of biological samples of RNA or DNA and append specific sequencing adaptors ...
Duplex sequencing is a library preparation and analysis method for next-generation sequencing ... Illumina sequencing adapters are used as an example following the ...