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"Analyzing gels and western blots with ImageJ". lukemiller.org "Quantification of Western Blots Using ImageJ". How to WESTERN-BLOT "Image Studio Lite Software". licor.com. Archived from the original on 3 March 2014 "ImageJ" "MCID Core Digital Imaging Software".
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging [ 6 ] to radiological image processing, [ 7 ] multiple imaging system data comparisons [ 8 ...
These assays are often followed by western blotting to assess protein abundance and can be analyzed using quantitative tools such as ImageJ. [2] Implementation. Cycloheximide chase assays have been conducted using a variety of cell types such as yeast and mammalian cell lines.
A western blot is used for the detection of specific proteins in complex samples. Proteins are first separated by size using electrophoresis before being transferred to an appropriate blotting matrix (usually polyvinylidene fluoride or nitrocellulose ) and subsequent detection with antibodies.
As stated on the official website, the primary focus is "life sciences", although Fiji provides many tools helping with scientific image analysis in general. [ 11 ] Fiji is most popular in the life sciences community, where the 3D Viewer [ 12 ] helps visualizing data obtained through light microscopy , and for which Fiji provides registration ...
Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.
The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins [1] by their ability to bind to specific oligonucleotide probes. Determination of molecular weight of proteins binding to DNA is also made possible by the technique.