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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
The inoculation needle after incineration is cooled down on an uninoculated region of the agar plate culture. [1] Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. [1] The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced.
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
Inoculation loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc. Sterilized by passing through a blue flame. Laminar flow cabinet: used to work aseptic Latex agglutination tiles: for serological analysis Lovibond comparator: a type of a colorimeter: McCartney's bottle or Flat medical bottle
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