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Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics studies microorganisms for different purposes. The microorganisms that are observed are bacteria and archaea. Some fungi and protozoa are also subjects used to study in this field.
Bacterial conjugation is the transfer of genetic material (plasmid) between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. [1] Discovered in 1946 by Joshua Lederberg and Edward Tatum, [ 2 ] conjugation is a mechanism of horizontal gene transfer as are transformation and transduction although ...
Transduction This is an illustration of the difference between generalized transduction, which is the process of transferring any bacterial gene to a second bacterium through a bacteriophage and specialized transduction, which is the process of moving restricted bacterial genes to a recipient bacterium. While generalized transduction can occur ...
Bacteria: Escherichia coli (E. coli), common Gram-negative gut bacterium widely used in molecular genetics. Main lab strain is 'K-12'. Bacillus subtilis, endospore forming Gram-positive bacterium. Main lab strain is '168'. Caulobacter crescentus, bacterium that divides into two distinct cells used to study cellular differentiation.
In genetics, attenuation is a regulatory mechanism for some bacterial operons that results in premature termination of transcription.The canonical example of attenuation used in many introductory genetics textbooks, [1] is ribosome-mediated attenuation of the trp operon.
Mobile genetic elements play a critical role in the spread of virulence factors, such as exotoxins and exoenzymes, among bacteria. Strategies to combat certain bacterial infections by targeting these specific virulence factors and mobile genetic elements have been proposed.
Among bacteria, E. coli is a particularly powerful model system to study the organization, recognition, and activation mechanism of replication origins. E. coli oriC comprises an approximately ~260 bp region containing four types of initiator binding elements that differ in their affinities for DnaA and their dependencies on the co-factor ATP .
Integrons are genetic structures in bacteria which express and are capable of acquiring and exchanging gene cassettes. The integron consists of a promoter, an attachment site, and an integrase gene that encodes a site-specific recombinase [2] There are three classes of integrons described. [1]