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Lactase (EC 3.2.1.108) is an enzyme produced by many organisms and is essential to the complete digestion of whole milk. It breaks down the sugar lactose into its component parts, galactose and glucose. Lactase is found in the brush border of the small intestine of humans and other mammals.
The lactase enzyme has two active sites which break down lactose. The first is at Glu1273 and the second is at Glu1749, which separately break down lactose into two separate kinds of molecules. [1] At least six mutations (single-nucleotide polymorphisms – SNPs) have been associated with lactase expression. [28]
(This enzyme digests many β-Galactosides, not just lactose. It is sometimes loosely referred to as lactase but that name is generally reserved for mammalian digestive enzymes that breaks down lactose specifically.) β-Galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule.
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Lactose intolerance does not cause damage to the gastrointestinal tract. [2] Lactose intolerance is due to the lack of the enzyme lactase in the small intestines to break lactose down into glucose and galactose. [3] There are four types: primary, secondary, developmental, and congenital. [1]
On the other hand, the enzyme corresponding to beta-galactosides is called beta-galactosidase; it breaks down the disaccharide lactose for instance, into its monosaccharide components, glucose and galactose. [1] Both varieties of galactosidase are categorized under the EC number 3.2.1.
The lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in E. coli and many other enteric bacteria.Although glucose is the preferred carbon source for most enteric bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available through the activity of β-galactosidase. [1]
In nature, lactose is found primarily in milk and milk products. Consequently, various food products made with dairy-derived ingredients can contain lactose. [14] Galactose metabolism, which converts galactose into glucose, is carried out by the three principal enzymes in a mechanism known as the Leloir pathway. The enzymes are listed in the ...