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Another requirement to effectively use 2D liquid chromatography, according to Bushey and Jorgenson, is to employ highly orthogonal techniques which means that the two separation techniques must be as different as possible. [17] Diagram of 1-dimensional LC. An example of the spectral results of this technique is also shown. [15]
Recrystallization: In analytical and synthetic chemistry work, purchased reagents of doubtful purity may be recrystallised, e.g. dissolved in a very pure solvent, and then crystallized, and the crystals recovered, in order to improve and/or verify their purity.
Copper-free click chemistry is a bioorthogonal reaction first developed by Carolyn Bertozzi as an activated variant of an azide alkyne Huisgen cycloaddition, based on the work by Karl Barry Sharpless et al. Unlike CuAAC, Cu-free click chemistry has been modified to be bioorthogonal by eliminating a cytotoxic copper catalyst, allowing reaction ...
Orthogonal tandem catalysis is a "one-pot reaction in which sequential catalytic processes occur through two or more functionally distinct, and preferably non-interfering, catalytic cycles". [7] This technique has been deployed in tandem alkane-dehydrogenation-olefin-metathesis catalysis [ 8 ] [ 9 ]
The axial pair lie along a common bond axis so that are related by a bond angle of 180°. The equatorial pair of ligands is situated in a plane orthogonal to the axis of the axial pair. Typically the bond distance to the axial ligands is longer than to the equatorial ligands.
Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure ().The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence.
Fitting of a noisy curve by an asymmetrical peak model, with an iterative process (Gauss–Newton algorithm with variable damping factor α).Curve fitting [1] [2] is the process of constructing a curve, or mathematical function, that has the best fit to a series of data points, [3] possibly subject to constraints.
An example of the 2D wavelet transform that is used in JPEG 2000. Cohen–Daubechies–Feauveau wavelets are a family of biorthogonal wavelets that was made popular by Ingrid Daubechies. [1] [2] These are not the same as the orthogonal Daubechies wavelets, and also not very similar in shape and properties. However, their construction idea is ...
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