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Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
Back-pressure is a useful diagnostic feature of problems with the chromatography column. [7] Rapid chromatography is favoured by columns packed with very small particles, which create high back-pressures. Column designers use "kinetic plots" to show the performance of a column at a constant back-pressure, usually selected as the maximum that a ...
Methanizer is an appliance used in gas chromatography (GC), which allows the user to detect very low concentrations of carbon monoxide and carbon dioxide. It consists of a flame ionization detector, preceded by a hydrogenating reactor, which converts CO 2 and CO into methane CH 4. Methanizers contain a hydrogenation catalyst to achieve this ...
A packed bed used to perform separation processes, such as absorption, stripping, and distillation is known as a packed column. [1] Columns used in certain types of chromatography consisting of a tube filled with packing material can also be called packed columns and their structure has similarities to packed beds.
Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
In industrial uses, sometimes a packing material is used in the column instead of trays, especially when low pressure drops across the column are required, as when operating under vacuum. This packing material can either be random dumped packing (1–3 in or 2.5–7.6 cm wide) such as Raschig rings or structured sheet metal.
Like other forms of chromatography, increasing the column length enhances resolution, and increasing the column diameter increases column capacity. Proper column packing is important for maximum resolution: An over-packed column can collapse the pores in the beads, resulting in a loss of resolution. An under-packed column can reduce the ...
Pages in category "Packing problems" The following 29 pages are in this category, out of 29 total. This list may not reflect recent changes. ...