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Centrifugation. Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. [1] The denser components of the mixture migrate away from the axis of the centrifuge, while the less dense components of the ...
In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate. Although often applied in biological analysis, differential centrifugation is a general technique also suitable ...
Clearing factor. In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time (in hours) required for sedimentation of a fraction with a known sedimentation coefficient (in svedbergs): The value of the clearing factor ...
A centrifuge is a device that uses centrifugal force to subject a specimen to a specified constant force - for example, to separate various components of a fluid. This is achieved by spinning the fluid at high speed within a container, thereby separating fluids of different densities (e.g. cream from milk) or liquids from solids.
The sedimentation coefficient is typically dependent on the concentration of the solute (i.e. a macromolecular solute such as a protein). Despite 80+ years of study, there is not yet a consensus on the way to perfectly model this relationship while also taking into account all possible non-ideal terms to account for the diverse possible sizes, shapes, and densities of molecular solutes. [2]
Ultracentrifuge. An ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1 000 000 g (approx. 9 800 km/s²). [1] There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge. Both classes of instruments find important uses in molecular ...
Centrifugal extractor. A centrifugal extractor —also known as a centrifugal contactor or annular centrifugal contactor —uses the rotation of the rotor inside a centrifuge to mix two immiscible liquids outside the rotor and to separate the liquids in the field of gravity inside the rotor. This way, a centrifugal extractor generates a ...
The gas centrifuge consists of a cylindrical rotor, a casing, an electric motor, and three lines for material to travel. The gas centrifuge is designed with a casing that completely encloses the centrifuge. [4] The cylindrical rotor is located inside the casing, which is evacuated of all air to produce a near frictionless rotation when operating.