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[1] [2] This process is called inoculation. The tool consists of a thin handle with a loop about 5 mm wide or smaller at the end. It was originally made of twisted metal wire (such as platinum, tungsten or nichrome), but disposable molded plastic versions are now common. The size of the loop determines the volume of liquid an inoculation loop ...
The inoculation needle after incineration is cooled down on an uninoculated region of the agar plate culture. [1] Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. [1] The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced.
However, during the initial heating, infectious material may be sprayed from the wire surface before it is killed, contaminating nearby surfaces and objects. Therefore, special heaters have been developed that surround the inoculating loop with a heated cage, ensuring that such sprayed material does not further contaminate the area.
Nichrome wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Petri dish/agar plate: to act as a supporting container to hold the culture medium in Platinum wire loop
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
Mechanical wire wrap tool Electrical wire wrap tool; pneumatic (air-powered) tools are also available. A "wire wrap tool" has two holes. The wire and 1 ⁄ 4 in (6.4 mm) of insulated wire are placed in a hole near the edge of the tool. The hole in the center of the tool is placed over the post. The tool is rapidly twisted.
Cell spreaders can be made from glass, plastic, or metal, and come in various shapes. A Drigalski spatula is a cell spreader consisting of a cylindrical rod or wire bent in the shape of a triangle with a handle. [1] [2] [3] Another variant is a rod bent in L-shape. [4] Extrusion molded versions can be T-shaped. [5]
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...