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Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid. Some liquid will remain inside the tip. The liquid remaining in the tip can be dispensed back into the original solution (#4) or thrown away. Release the pipette thumb to the ready ...
Release the pipette knob slowly to the ready position. Do not remove the tip from the solution. Repeat this process (step 3 and 4) to rinse the tip until the interior wall of the tip is clear. Remove the tip from the solution. Press the pipette knob to the second stop, and completely empty the tip. Release the pipette knob to the ready position.
Generally, a pipette has optimal accuracy from 35-100% of its nominal volume, and should not be used below 10% of that volume. [5] For example, using a 1,000 μl pipette for 50 μl of liquid is not ideal, and using a 100 μl pipette will give better results. [6] Other factors like tip angle and immersion depth may also impact accuracy ...
For example, a motorized pipette controller can aid liquid aspiration or dispensing using volumetric pipettes or graduated pipettes; [13] a tablet can interact in real-time with the pipette and guide a user through a protocol; [14] and a pipette station can help to control the pipette tip immersion depth and improve ergonomics. [15]
Less commonly, some TD pipettes are made "to contain" as per manufacturer and made to be blown out. [10] [11] A set of two rings printed on the upper end of the pipette indicate that it is a "blow out" type and should be blown using a rubber bulb. Do not blow the solution out if the pipette has no rings on the upper end. [3]
Different sizes of rubber bulb, flip style pipette filler and wheel style pipette filler. The rubber bulbs can also be replaced with wheel style pipette filler or flip style pipette filler. The flip style pipette fillers have two valves system and have a removable top valve which makes cleaning easy and it can be used with one hand. [3] The ...