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Ligation-independent cloning (LIC) is a form of molecular cloning that can be performed without the use of restriction endonucleases or DNA ligase.The technique was developed in the early 1990s as an alternative to restriction enzyme/ligase cloning. [1]
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The U.S. state of Washington first required its residents to register their motor vehicles in 1905. Registrants provided their own license plates for display until 1915, when the state began to issue plates.
In 1956, the United States, Canada, and Mexico came to an agreement with the American Association of Motor Vehicle Administrators, the Automobile Manufacturers Association and the National Safety Council that standardized the size for license plates for vehicles (except those for motorcycles) at 6 inches (15 cm) in height by 12 inches (30 cm) in width, with standardized mounting holes. [2]
Dilithium acetylide is an organometallic compound with the formula Li 2 C 2.It is typically derived by double deprotonation of acetylene. X-ray crystallography confirms the presence of C≡C subunits attached to lithium, resulting in a polymeric structure. [3]
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