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Enter one or more queries in the top text box and one or more subject sequences in the lower text box. Then use the BLAST button at the bottom of the page to align your sequences. To get the CDS annotation in the output, use only the NCBI accession or gi number for either the query or subject.
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.
This tutorial demonstrates how use Protein BLAST to align and compare two or more amino acid sequences.
BLASTp (Protein BLAST): compares one or more protein query sequences to a subject protein sequence or a database of protein sequences. This is useful when trying to identify a protein (see From sequence to protein and gene below).
Select the Blast tab of the toolbar to run a sequence similarity search with the BLAST (Basic Local Alignment Search Tool) program: Enter either a protein or nucleotide sequence (raw sequence or fasta format) or a UniProt identifier into the form field. Click the Blast button.
Comparing two or more protein sequences using BLASTp. Tutorial Sequences. Identifying a gene. This section of the tutorial explains how to take an unknown sequence and identify from which organism and where in the genome it originates. Navigate to BLAST hosted by the National Center for Biotechnology Information (NCBI).
The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between protein or nucleotide sequences. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches.
In a BLAST search form, the “Blast 2 sequences” checkbox (A) activates the “Align Two Sequences” function and dis-plays the subject sequence input box (B) while removing the elements pertaining to database selection. The “Align Two Sequences” also adds a new set of parameters for fine tuning searches:
How to: Submit multiple query sequences in a single BLAST search. Starting with ... THE NCBI WEB BLAST INTERFACE. Choose the appropriate BLAST service from the BLAST Homepage. Enter NCBI sequence identifiers (accession numbers, gi numbers) or FASTA-formatted sequences in the appropriate text box.
Basic Local Alignment Search Tool (BLAST) [1, 2] is a sequence similarity search program that can be used to quickly search a sequence database for matches to a query sequence. Several variants of BLAST exist to compare all combinations of nucleotide or protein queries against a nucleotide or protein database.