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Preclinical imaging is the visualization of living animals for research purposes, [1] such as drug development. Imaging modalities have long been crucial to the researcher in observing changes, either at the organ, tissue, cell, or molecular level, in animals responding to physiological or environmental changes.
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
MINFLUX, or minimal fluorescence photon fluxes microscopy, is a super-resolution light microscopy method that images and tracks objects in two and three dimensions with single-digit nanometer resolution. [1] [2] [3]
Glass chart. A 1951 USAF resolution test chart is a microscopic optical resolution test device originally defined by the U.S. Air Force MIL-STD-150A standard of 1951. The design provides numerous small target shapes exhibiting a stepped assortment of precise spatial frequency specimens.
Preclinical SPECT is a quantitative imaging modality. The uptake of SPECT tracers in organs (regions) of interest can be calculated from reconstructed images. The small size of laboratory animals diminishes the photon’s attenuation in the body of the animal (compared to one in human-sized objects).
Imaging lenses and digital cameras (CCD or CMOS) are used to produce the final image. Live video processing can also be performed to enhance contrast during fluorescence detection and improve signal-to-background ratio. In recent years a number of commercial companies have emerged to offer devices specializing in fluorescence in the NIR ...
In drug development, preclinical development (also termed preclinical studies or nonclinical studies) is a stage of research that begins before clinical trials (testing in humans) and during which important feasibility, iterative testing and drug safety data are collected, typically in laboratory animals.
The first intravascular in vivo use in a preclinical model was reported in 1994 [6] and first in human, clinical imaging in 2003. [7] The first OCT imaging catheter and system was commercialized by LightLab Imaging, Inc., a company based in Massachusetts formed following a technology transfer in 1997 from Fujimoto's lab (MIT).
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