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Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL pipette has a tolerance of ±0.006 mL, while a 50-mL pipette ...
A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision , from single piece glass pipettes to more complex adjustable or ...
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]
With regard to the volume transferred, the smallest pipette that can handle the required volume should be selected. This is important because accuracy decreases when the set volume is close to the pipette's minimum capacity. Generally, a pipette has optimal accuracy from 35-100% of its nominal volume, and should not be used below 10% of that ...
Compared to a volumetric pipette, a burette has similar precision if used to its full capacity, but as it is usually used to deliver less than its full capacity, a burette is slightly less precise than a pipette. [6] The burette is used to measure the volume of a dispensed substance, but is different from a graduated cylinder as its graduations ...
Dip the tip into the solution to a certain depth according to the volume set. Afterwards slowly release the pipette knob till the starting position (#2). Withdraw the tip from the liquid. Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid.
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