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A DNA construct is an artificially-designed segment of DNA borne on a vector that can be used to incorporate genetic material into a target tissue or cell. [1] A DNA construct contains a DNA insert, called a transgene , delivered via a transformation vector which allows the insert sequence to be replicated and/or expressed in the target cell.
The BASIC assembly standard provides several linkers embedded with RBS of different strengths. Similarly to facilitate the construction of fusion proteins containing multiple protein domains, several fusion linkers were also designed to allow for full read-through of the DNA construct.
Nucleic acid design can be used to create nucleic acid complexes with complicated secondary structures such as this four-arm junction. These four strands associate into this structure because it maximizes the number of correct base pairs, with A's matched to T's and C's matched to G's.
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DNA replication also works by using a DNA template, the DNA double helix unwinds during replication, exposing unpaired bases for new nucleotides to hydrogen bond to. Gene synthesis, however, does not require a DNA template and genes are assembled de novo. DNA synthesis occurs in all eukaryotes and prokaryotes, as well as some viruses. The ...
Game testing, also called quality assurance (QA) testing within the video game industry, is a software testing process for quality control of video games. [ 1 ] [ 2 ] [ 3 ] The primary function of game testing is the discovery and documentation of software defects .
DNA-directed RNA interference (ddRNAi) is a gene-silencing technique that utilizes DNA constructs to activate a cell's endogenous RNA interference (RNAi) pathways. DNA constructs are designed to express self-complementary double-stranded RNAs, typically short-hairpin RNAs (shRNA), that bring about the silencing of a target gene or genes once processed. [1]
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