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A quadrupole time-of-flight hybrid tandem mass spectrometer. Tandem mass spectrometry, also known as MS/MS or MS 2, is a technique in instrumental analysis where two or more stages of analysis using one or more mass analyzer are performed with an additional reaction step in between these analyses to increase their abilities to analyse chemical samples. [1]
In mass spectrometry, data-independent acquisition (DIA) is a method of molecular structure determination in which all ions within a selected m/z range are fragmented and analyzed in a second stage of tandem mass spectrometry. [1] [2] Tandem mass spectra are acquired either by fragmenting all ions that enter the mass spectrometer at a given ...
Both, the first mass analyzer and the collision cell are continuously exposed to ions from the source, in a time independent manner. [4] It is once the ions move into the third mass analyzer that time dependence becomes a factor. [4] The first quadrupole mass filter, Q1, is the primary m/z selector after the sample leaves the ionization source.
In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. In the old days, this was accomplished by the Edman degradation ...
The tandem mass spectrometry data on over 930,000 molecular standards (as of January 2024) [33] [36] is provided to facilitate the identification of chemical entities from tandem mass spectrometry experiments. [37] In addition to the identification of known molecules it is also useful for identifying unknowns using its similarity searching ...
The structures of TMT tags are publicly available through the unimod database at unimod.org and hence, mass spectrometry software such as Mascot are able to account for the tag masses. Additionally, as of version 2.2, Mascot has the capability to quantify using TMT and other isobaric mass tags without the use of additional software. Intuitively ...
Selected reaction monitoring (SRM), also called multiple reaction monitoring (MRM), is a method used in tandem mass spectrometry in which an ion of a particular mass is selected in the first stage of a tandem mass spectrometer and an ion product of a fragmentation reaction of the precursor ions is selected in the second mass spectrometer stage ...
Tandem mass spectrometry (MS/MS) database of over 930,000 (as of December 2023) [59] metabolites and other molecular entities, generated from high resolution quadrupole time-of-flight tandem mass spectrometry. [60] It also includes, ion mobility, [61] neutral loss, [62] and LC/MS data. [63] [64] mineXpert Open source GPL