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Isomorphous replacement (IR) is historically the most common approach to solving the phase problem in X-ray crystallography studies of proteins. For protein crystals this method is conducted by soaking the crystal of a sample to be analyzed with a heavy atom solution or co-crystallization with the heavy atom.
Single-wavelength anomalous diffraction (SAD) is a technique used in X-ray crystallography that facilitates the determination of the structure of proteins or other biological macromolecules by allowing the solution of the phase problem.
The main difference between the effects of single and multiple scattering is that single scattering can usually be treated as a random phenomenon, whereas multiple scattering, somewhat counterintuitively, can be modeled as a more deterministic process because the combined results of a large number of scattering events tend to average out.
Small-angle X-ray scattering (SAXS) is a small-angle scattering technique by which nanoscale density differences in a sample can be quantified. This means that it can determine nanoparticle size distributions, resolve the size and shape of (monodisperse) macromolecules, determine pore sizes and characteristic distances of partially ordered materials. [1]
The dynamical theory of diffraction considers the wave field in the periodic potential of the crystal and takes into account all multiple scattering effects. Unlike the kinematic theory of diffraction which describes the approximate position of Bragg or Laue diffraction peaks in reciprocal space , dynamical theory corrects for refraction, shape ...
Small-angle scattering (SAS) is a scattering technique based on deflection of collimated radiation away from the straight trajectory after it interacts with structures that are much larger than the wavelength of the radiation. The deflection is small (0.1-10°) hence the name small-angle. SAS techniques can give information about the size ...
In combination with the straightforward scattering geometry, where all relevant information is contained in a single scattering image, in-situ and real-time experiments are facilitated. Specifically self-organization during MBE growth [ 2 ] and re-organization processes in block copolymer films under the influence of solvent vapor [ 3 ] have ...
This program calculates the scattering, absorption, and attenuation parameters, as well as the angular scattering patterns of a single coated sphere according to Aden-Kerker theory. 2007 L. Liu, H. Wang, B. Yu, Y. Xu, J. Shen [15] C: Unknown Light scattering by a coated sphere (extinction efficiency, scattering efficiency, light scattering ...