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Paper chromatography is a useful technique because it is relatively quick and requires only small quantities of material. Separations in paper chromatography involve the principle of partition. In paper chromatography, substances are distributed between a stationary phase and a mobile phase.
used to separate particles dispersed in a liquid according to their molecular mass: Electrophoresis apparatus: used to detect and classify serum proteins or proteins from any other source; also used for DNA separation Chromatography: • Gas chromatography or Gas liquid chromatography (GLC) • Planar chromatography • Paper chromatography
Also used for DNA separation. Chemical laboratory apparatus: for Chemical tests: Chromatography: One of the basic modern "chemical examination" of body fluids and viscera;video links for details •Gas Chromatography or Gas Liquid Chromatography(GLC)-do- •Planar Chromatography-do- •Paper Chromatography-do- •Thin layer chromatography-do-
Paper chromatography is a technique that involves placing a small dot or line of sample solution onto a strip of chromatography paper. The paper is placed in a container with a shallow layer of solvent and sealed. As the solvent rises through the paper, it meets the sample mixture, which starts to travel up the paper with the solvent.
There is one big drawback in using chromatography, which has to do with the economics of the process. Although the method was efficient from the processing aspect, acquiring the necessary equipment is a big task. Large machinery is necessary, and for a long time the lack of equipment availability was not conducive to its widespread use.
Chromatography separates dissolved substances by different interaction with (i.e., travel through) a material. High-performance liquid chromatography (HPLC) Thin-layer chromatography (TLC) Countercurrent chromatography (CCC) Droplet countercurrent chromatography (DCC) Paper chromatography; Ion chromatography; Size-exclusion chromatography (SEC)
Uses Flow cytometer: used for automated cell counting as in total blood count, differential count, etc. Tissue bath or organ bath or Dale's apparatus: used in full tissue experiments, for example using guinea pig ileum mainly used in pharmacology for application of drugs to these tissues. Sahli Haemoglobinometer
Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups. For reversed phase, alkyl hydrocarbons are the preferred stationary phase; octadecyl (C18) is the most common stationary phase, but octyl (C8) and butyl (C4) are also used in some applications. The designations for ...